![]() Accordingly, a much better understanding of this process should enable researchers to realize the fruiting body formation of S. Yang et al.found that vitamin B6 metabolism, glycine metabolism, and cystathionine lyase, which might play an important role during light-inducedprimordia formation 9. latifolia, little research had been conducted on how the developing fruiting body is controlled because suitable analytical tools and the prerequisite genetic information were unavailable 8. For some valuable and rare mushroom species, particularly S. The formation stage of fruiting body is the most complicated transformations in the cyclogeny of mushrooms, co-regulated by genetic, physiological, growth, as well as environmental factors 7.ĭue to the lack of knowledge about the key mechanism underpinning fruiting body formation, numerous edible mushrooms still cannot be readily cultivated via artificial techniques. Our current understanding of the process entailing fruiting body initiation and development is nascent, and how the mycelium forms the fruting body is a prominent subject in the field of fungi molecular biology, especially concerning Coprinopsis cinerea, Schizophyllum commune, and certain non-model species, such as Agaricus bisporus, Flammulina velutipes, and Boletus edulis 5, 6. latifolia based on a morphological comparison and molecular phylogeny analysis 4. crispa of Asia present significant differences from conspecific strains originating from Europe and North America, so the Asian isolates were re-classified as S. crispa until phylogenic evidence from three nuclear rDNA marker genes and mitochondrial gene confirmed S. latifolia had been wrongly recognized as S. In the last several years, however, there has been successful progress on the artificial breeding of Sparassis in Japan, China, and Korea 2. Among members of this genus, the only species that current can be artificially cultivated is S. Similar content being viewed by othersĬauliflower mushroom ( Sparassis) is widely known for its characteristic fruiting body that appears similar to cauliflower, and is recognized as an edible mushroom with numerous medicinal properties as well 1. latifolia, and advances our knowledge about mushroom morphogenesis. These findings provide crucial candidate genes and pathways related to primordium differentiation and development in S. ![]() Further, our research revealed the PI3K-Akt signaling pathway related to cell proliferation could play crucial functions during the development of SP and SPD. ![]() The MAPK and mTOR signal transduction pathways underwent significant adjustments during the SM to SP transition. KEGG pathway enrichment analysis of DEGs (differentially expressed genes) indicated primordium initiation was significantly related to 66 pathways, such as “Ribosome”, “metabolism of xenobiotics by cytochrome P450”, and “glutathione metabolism” (among others). KEGG and KOG analyses respectively mapped 32,765 unigenes to 202 pathways and 19,408 unigenes to 25 categories. The de novo assembly generated a total of 48,549 unigenes, of which 71.53% (34,728) unigenes could be annotated by at least one of the KEGG (Kyoto Encyclopedia of Genes and Genomes), GO (Gene Ontology), and KOG (Eukaryotic Orthologous Group) databases. To investigate this process, 4.8 × 10 8 RNA-Seq reads were acquired from three stages: hyphal knot (SM), primordium (SP), and primordium differentiation (SPD). The genes associated with fruiting body formation of Sparasis latifolia are valuable for improving mushroom breeding. ![]()
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